See Easy Ways to Detect TSV Disease in Shrimp Correctly!

TSV disease in shrimp or often called red tail shrimp disease is a shrimp disease that can cause significant mortality. TSV attacks on shrimp generally occur at the age of 14-40 days after stocking the shrimp seeds in ponds with a mortality rate reaching 9%. In addition, adult shrimp can also be attacked by TSV disease with a low mortality rate. 

There are two phases of TSV infection in shrimp, namely the acute phase (mass shrimp death) and the chronic phase (can survive but can carry carrier TSV disease). As a result, TSV disease in shrimp has a detrimental effect on your shrimp culture. Come on, read more in this article!

What is TSV in Shrimp?

Taura Syndrome Disease (TSV) is a disease Ribonucleic Acid (RNA) shrimp which was first discovered in 1992 and comes from shrimp farming in Ecuador. Shrimp TSV has spread to large shrimp-producing countries through the transfer of fry and broodstock for aquaculture purposes or frozen shrimp feed products. TSV is classified as one of the most dangerous shrimp diseases.

Symptoms of TSV Disease

Here are some symptoms that can indicate shrimp experiencing TSV in pond ponds:

1. Shrimp lethargic / not actively moving
2. Reduced appetite for shrimp
3. Shrimp gather at the edge of the pond pond
4. Sudden death in post larval and juvenile stages
5. Shrimp body pale red
6. Shrimp tail is red
7. Soft shrimp shell
8. The existence of black spots / melanization around the body of the shrimp

These symptoms will then attack the most important parts of the shrimp, namely the gills, kidneys, brain and liver of the shrimp. This is what can cause the death of shrimp quickly in one shrimp population

Causes of TSV in Shrimp

TSV in shrimp occurs due to various factors, such as high shrimp density and unstable temperature so that the shrimp become stressed. TSV is a moderately dangerous virus that is classified as class 1 Quarantine Fish Pest and Disease (HPIK). Taura Syndrome Virus (TSV) attacks shrimp aged 14-40 days after stocking in aquaculture ponds. 

How to Detect TSV in Shrimp

Detecting TSV in shrimp can be done by PCR test or Polymerase Chain Reaction. PCR is the fastest and easiest way to detect TSV. The PCR method can detect TSV disease molecularly. The way the PCR test finds TSV is by multiplying specific DNA fragments.

There are 5 ways to test using the PCR method, namely sample necropsy, extraction, amplification, electrophoresis, and visualization of the results:

Necropsy can be done by taking samples of target organs such as gills, uropods, cuticles, and shrimp swimming legs. 

1. Next, the extraction was carried out to separate the DNA/RNA components of the cells silica extraction kit.
2. The extraction results are then amplified using a machine Polymerase Chain Reaction. This extraction consists of 5 processes namely pre-denaturation, denaturation, annealing, extension, and final extension.
3. The results of the amplification will be carried out by electrophoresis. The purpose of electrophoresis is to determine the size and shape of a particle, both DNA, RNA and protein
4. The results will be socialized on the UV Transluminator computer.

PCR can prevent and minimize TSV infection in shrimp culture ponds. Good and appropriate monitoring and PCR methods can determine the process of maintaining shrimp culture until maximum harvest.

Atasi Penyakit TSV dengan Berkonsultasi di Konsultasi Budidaya! GRATIS!